Extension Ag Update
July/August 2004
Articles Research Resources Internet Links Ag Facts Education

Research

Insect scent preferences offer clues to mating choices and organic pest controls

Just like humans, certain flies need to be "in the mood" before they mate. And nothing creates a better mood for apple flies than the scent of a nice apple tree, while hawthorn flies prefer an essence of hawthorn in the air before finding a mate. These mating preferences are strange because technically, both flies are the same species; they are known by different names depending on which tree they are infesting at the time. Stewart Berlocher, professor of entomology in the University of Illinois College of Liberal Arts and Science, and colleagues at Notre Dame and Cornell, have discovered that it is a peculiar preference for the trees' scents that keeps the flies separated. The discovery of the flies' odor partialities suggests the decision to mate may be a matter of scent. This research could aid in organic pest control. The apple maggot, a notorious pest of apple orchards all over the U.S., could be deterred by introducing essence of hawthorn into the orchards.

The scientists made their discovery through a process called solid phase microextraction. This process allowed them to isolate volatiles, key components of fruit odors that the flies use for host plant identification. After running a series of behavioral and field experiments, they discovered that the apple and hawthorn flies were only attracted by one of the scents. It was previously theorized that geographical isolation was needed to keep populations from mixing, but the apple flies and the hawthorn flies stuck to their favorite orchards and groves and have done so for 150 years. This scent preference is so strong that the flies tend not to interbreed, which is one step in the development of a new species. (Source: University of Illinois College of Liberal Arts and Science News)

Chicken litter linked to antibiotic-resistance

Phil Williams, University of Georgia, College of Agriculture and Environmental Sciences, College of Arts and Sciences, 706-542-8501, Phil@franklin.uga.edu

Scientists may need to reexamine assumptions about the spread of antibiotic-resistant genes, according to a new study by researchers at the University of Georgia. They found that poultry litter -a ubiquitous part of large broiler operations - harbors a vastly larger number of microbial agents that collect and express resistance genes than was previously known. The study shows that waste left behind by flocks raised in industrial chicken houses is rich in genes called integrons that promote the spread and persistence of clusters of varied antibiotic resistance genes.

"We were surprised to find a vastly greater pool of these multi-resistance clustering agents than anyone had suspected before," said Anne Summers, a UGA microbiologist who led the study. "Finding such a huge reservoir of integrons explains a long-standing puzzle about how clusters of resistance genes spread so rapidly and persist in bacterial communities even after antibiotic use concludes." Other authors of the paper included Sobhan Nandi, a postdoctoral associate in the UGA department of microbiology, and John Maurer and Charles Hofacre of the department of avian medicine in UGA's College of Veterinary Medicine. Maurer also holds an appointment with the UGA College of Agricultural and Environmental Sciences' Center for Food Safety in Griffin.

Antibiotic resistance is a serious and growing problem for farm animal operations and human health. Antibiotic use to treat disease and increase feed efficiency has been a common part of industrial farms for more than half a century. When antibiotic-resistant bacteria began to show up in hospitals in the 1950s, researchers initially believed that simply restricting the use of antibiotics on farms could reduce the prevalence of antibiotic resistance among humans. "Over the past 30 years, we have learned this hope was unrealistic because we share both pathogenic and benign bacteria with other humans and animals," said Summers, "and because bacteria transfer genes among themselves."

At the heart of the multi-resistance problem are integrons, which scientists until now have exclusively studied in such pathogenic bacteria as Salmonella and E. coli. The UGA team wondered, however: Does the poultry production environment also harbor integrons that assemble these large clusters of distinct resistance genes? To find out, samples of poultry litter from Georgia broiler houses were collected regularly over a 13-week period. Litter begins as a bedding material of softwood shavings placed in commercial broiler houses before chicks are brought to it. By the time the flock is harvested, the shavings have become mixed with chicken feces, uric acid, skin, feathers, insects and small invertebrates. Rich in minerals, poultry litter is often recycled for fertilizer and other uses.

What the researchers discovered was startling: One integron type, called intl1 (typically found in E. coli and Salmonella) was up to 500 times more abundant than these bacteria themselves were in litter. A bit of microbial sleuthing revealed that integrons are also carried by so-called Gram positive bacteria that are much more abundant in litter than the E. coli-type bugs, called Gram negative bacteria. "The fact that integron genes in the Gram positive bacteria are identical to those of E. coli indicates they are being actively exchanged among these otherwise unrelated bacteria," said Summers. "Just as intriguing, integrons and resistance genes were abundant regardless of antibiotic use on the farms, suggesting that, once acquired, integrons are inherently stable, even without continual exposure to antibiotics."

The study has several significant implications, said Summers. Most studies of antibiotic resistance have been done in hospital settings, and until recently, much less work has been done on the real-world ecology of systems that create multiple-resistant clusters. Knowledge about how antibiotic resistances spread from animals to humans is at present sketchy; however, since humans and their pets are "colonized" by similar bacteria, it is reasonable to think we and our companion animals also harbor such multi-resistance gene clusters that are enriched when we take an antibiotic ourselves or treat our pets.

Humans and animals have billions of bacteria in and on their bodies at any time, and even if resistance to a single antibiotic arises in a few of them through mutation, there are still several other antibiotics that can eliminate them. But if bacteria in the same environment are already equipped with clusters of genes conferring resistance to many antibiotics and can readily exchange these clusters, then the treatment options are limited. "That's what we have today, and the surprising abundance of integrons in the environment is a key as to why we have this problem," said Summers. The discovery is now leading Summers and her UGA colleagues to see whether these resistance-gene-clustering systems are present in previously unrecognized reservoirs in companion animals and humans. The results will change our understanding of where resistance to new antibiotics will develop and how fast and how far it will spread and have implications for all antibiotic use, not just that in agriculture. The research was supported by a grant from the National Research Initiative of the U.S. Department of Agriculture and made possible by four anonymous poultry producing companies that afforded free access to their facilities for sample collection.